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... > Investigative Techniques > Chemistry Techniques, Analytical > Chromatography > Chromatography, Liquid > Chromatography, Affinity > Tandem Affinity Purification

Preferred term

Tandem Affinity Purification  

Type

  • Topical Descriptor

Broader concept

Entry terms

  • Affinity Chromatography, Tandem
  • Chromatography, Tandem Affinity
  • Tandem Affinity Chromatographies
  • Tandem Affinity Chromatography

Scope note

  • A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES that have been assembled with a tagged component (bait) that interacts with other partners in the complex. The interacting bait component is labeled with a dual-affinity tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification of only the partners of the complex interacting with the bait by affinity chromatography.

History note

  • 2019

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URI

http://www.yso.fi/onto/mesh/D000078791

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