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Concept information

Preferred term

Flow Cytometry  

Type

  • Topical Descriptor

Entry terms

  • Cytofluorometries, Flow
  • Cytofluorometry, Flow
  • Cytometries, Flow
  • Cytometry, Flow
  • Flow Cytofluorometries
  • Flow Cytofluorometry
  • Flow Cytometries
  • Flow Microfluorimetry
  • Flow Microfluorometries
  • Flow Microfluorometry
  • Microfluorimetry, Flow
  • Microfluorometries, Flow
  • Microfluorometry, Flow

Note

  • flow cell sorting & FLUORESCENCE-ACTIVATED CELL SORTING: coord with CELL SEPARATION

Scope note

  • Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

History note

  • 82; FLOW MICROFLUORIMETRY was see FLUOROIMMUNOASSAY 1989-90

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URI

http://www.yso.fi/onto/mesh/D005434

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