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Investigative Techniques > Genetic Techniques > In Situ Nick-End Labeling

Preferred term

In Situ Nick-End Labeling  

Type

  • Topical Descriptor

Broader concept

Entry terms

  • In Situ Nick End Labeling
  • In Situ Nick End-Labeling
  • In Situ Nick End Labelling
  • In Situ Nick End-Labelling
  • In Situ Nick-End Labelling
  • Nick End Labeling, In Situ
  • Nick End-Labeling, In Situ
  • Nick-End Labeling, In Situ
  • Nick End Labelling, In Situ
  • Nick End-Labelling, In Situ
  • Nick-End Labelling, In Situ
  • TUNEL

Scope note

  • An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.

History note

  • 99

In other languages

URI

http://www.yso.fi/onto/mesh/D020287

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