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Investigative Techniques > Genetic Techniques > Nucleic Acid Amplification Techniques > Ligase Chain Reaction

Preferred term

Ligase Chain Reaction  

Type

  • Topical Descriptor

Broader concept

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Scope note

  • A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.

History note

  • 2001

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URI

http://www.yso.fi/onto/mesh/D022521

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