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Investigative Techniques > Microscopy > Nonlinear Optical Microscopy > Microscopy, Fluorescence, Multiphoton
... > Diagnosis > Diagnostic Techniques and Procedures > Diagnostic Imaging > Microscopy > Nonlinear Optical Microscopy > Microscopy, Fluorescence, Multiphoton
... > Technology, Industry, and Agriculture > Engineering > Optics and Photonics > Microscopy > Nonlinear Optical Microscopy > Microscopy, Fluorescence, Multiphoton
... > Natural Science Disciplines > Physics > Optics and Photonics > Microscopy > Nonlinear Optical Microscopy > Microscopy, Fluorescence, Multiphoton
Investigative Techniques > Microscopy > Microscopy, Fluorescence > Microscopy, Fluorescence, Multiphoton
... > Diagnosis > Diagnostic Techniques and Procedures > Diagnostic Imaging > Microscopy > Microscopy, Fluorescence > Microscopy, Fluorescence, Multiphoton
... > Technology, Industry, and Agriculture > Engineering > Optics and Photonics > Microscopy > Microscopy, Fluorescence > Microscopy, Fluorescence, Multiphoton
... > Natural Science Disciplines > Physics > Optics and Photonics > Microscopy > Microscopy, Fluorescence > Microscopy, Fluorescence, Multiphoton

Preferred term

Microscopy, Fluorescence, Multiphoton  

Type

  • Topical Descriptor

Broader concept

Entry terms

  • Excitation Microscopies, Multiphoton
  • Excitation Microscopy, Multiphoton
  • Fluorescence Microscopy, Multiphoton
  • Microscopies, Multiphoton Excitation
  • Microscopy, Multiphoton Excitation
  • Microscopy, Multiphoton Fluorescence
  • Multiphoton Excitation Microscopies
  • Multiphoton Excitation Microscopy
  • Multiphoton Fluorescence Microscopy

Scope note

  • Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.

History note

  • 2003

In other languages

URI

http://www.yso.fi/onto/mesh/D036641

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