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... > Investigative Techniques > Chemistry Techniques, Analytical > Photometry > Luminescent Measurements > Fluorometry > Fluorescence Recovery After Photobleaching

Preferred term

Fluorescence Recovery After Photobleaching  

Type

  • Topical Descriptor

Broader concept

Entry terms

  • Fluorescence Photobleaching Recovery
  • FRAP (Fluorescence Recovery After Photobleaching)
  • FRAPs (Fluorescence Recovery After Photobleaching)

Scope note

  • A method used to study the lateral movement of MEMBRANE PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).

History note

  • 2003

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URI

http://www.yso.fi/onto/mesh/D036681

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